diff --git a/pkgs/development/python-modules/primer3/default.nix b/pkgs/development/python-modules/primer3/default.nix
new file mode 100644
index 00000000000..e8aa83ed94d
--- /dev/null
+++ b/pkgs/development/python-modules/primer3/default.nix
@@ -0,0 +1,32 @@
+{ lib
+, buildPythonPackage
+, fetchFromGitHub
+, cython
+, click
+}:
+
+buildPythonPackage rec {
+  pname = "primer3";
+  version = "0.6.1";
+
+  src = fetchFromGitHub {
+    owner = "libnano";
+    repo = "primer3-py";
+    rev = version;
+    sha256 = "1glybwp9w2m1ydvaphr41gj31d8fvlh40s35galfbjqa563si72g";
+  };
+
+  nativeBuildInputs = [ cython ];
+
+  # pytestCheckHook leads to a circular import issue
+  checkInputs = [ click ];
+
+  pythonImportsCheck = [ "primer3" ];
+
+  meta = with lib; {
+    description = "Oligo analysis and primer design";
+    homepage = "https://github.com/libnano/primer3-py";
+    license = with licenses; [ gpl2Only ];
+    maintainers = with maintainers; [ fab ];
+  };
+}
diff --git a/pkgs/top-level/python-packages.nix b/pkgs/top-level/python-packages.nix
index 8b5f6ec0d60..c7dfc66e87d 100644
--- a/pkgs/top-level/python-packages.nix
+++ b/pkgs/top-level/python-packages.nix
@@ -4899,6 +4899,8 @@ in {
   else
     callPackage ../development/python-modules/prettytable/1.nix { };
 
+  primer3 = callPackage ../development/python-modules/primer3 { };
+
   priority = callPackage ../development/python-modules/priority { };
 
   prison = callPackage ../development/python-modules/prison { };