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| File Name : COMBRISK.ASC | Online Date : 01/15/96 |
| Contributed by : InterNet | Dir Category : ECOLOGY |
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Combined, Environmental Risks Deadly, Researchers Learn
Acidic water and ultraviolet light in combination damage the embryos of frogs
far more seriously when acting together than when acting separately, according
to a laboratory study at the University of California, Santa Cruz. This
finding has major implications for understanding environmental threats to many
species.
Researchers exposed the eggs of a common species of frog to various levels of
acidity and UV-B radiation, the most harmful form of ultraviolet light. By
themselves, even the most acidic water or the most severe UV-B radiation did
not kill many embryos. But when the two environmental assaults were combined,
as many as half of the eggs failed to hatch.
Ecologists should look for similar interactions in nature as they try to
explain why many populations of amphibians around the world are dying out, the
study's authors say. Stresses such as habitat loss, a thinning ozone layer,
acid rain, pesticides, disease, and global climate change may combine to wreak
more havoc on frogs, toads and salamanders than any one factor could by
itself. Indeed, some scientists now suspect that for any given group of
amphibians in trouble, two or more such factors may interact synergistically -
that is, the combined effects of the stresses are far greater than the sum of
their individual effects.
"People have been looking for a smoking gun to explain the amphibian die-offs,
and there isn't just one smoking gun," says Michael Soule, coauthor of the
study and professor emeritus of environmental studies at UCSC. "There's quite
an armamentarium out there. We're just beginning to look at the interactions
among these possible causes."
The UCSC group published its work in the October 1995 issue of the scientific
journal Conservation Biology. First author was biologist Leslie Long, who
completed the study for her master's thesis in marine sciences. Coauthors were
former UCSC undergraduate Loralei Saylor, now at the Arizona Department of
Game & Fish, and Soule.
"We're not trying to say that a combination of UV radiation and low pH is the
answer for why a particular amphibian population is declining, or even that
this is happening now in the field," says Long. "The main message is that
synergisms can be important and are worth looking for. We may miss a lot if we
only look at each factor in isolation."
Another recent study supports Long's view. Ecologists Joseph Kiesecker and
Andrew Blaustein at Oregon State University found that a synergism between UV-
B radiation and a fungal disease can kill embryos of the Cascades frog and the
western toad. The combined effects of ultraviolet light and infection by the
fungus magnified the harm done to the eggs beyond the sum of the individual
effects of each factor. The work appeared in the November 21 issue of the
Proceedings of the National Academy of Sciences.
In 1994, Blaustein's group also showed that UV-B light by itself can kill frog
and toad embryos in the field. "Both our new paper and the Long paper show
that we should look not just for single effects, but also for combinations,"
Blaustein says. "We don't think low pH is a factor in Oregon, but it may
interact with UV radiation in other parts of the country. UV is important
here, but it doesn't play a big role in the tropics. There will not be
a single global cause."
For their study, Long and her colleagues used three levels of acidic water (pH
readings of 6.0, 5.0, and 4.5) and three levels of ultraviolet light: no UV-B
(as a control), a "normal" level for high elevations, and a level forecast if
the ozone layer continues to thin. The team exposed several dozen eggs of the
leopard frog, Rana pipiens, to one of the nine possible combinations of
factors. About one-quarter of the embryos died under the conditions of lowest
pH and moderate UV, while half died when subjected to the lowest pH and the
highest UV. In all other treatments - including the lowest pH by itself and
the highest UV by itself - at least 90 percent of the eggs hatched.
Several caveats apply, the authors note. The highest level of UV-B radiation
simulated a future earth with an ozone layer 30 percent thinner than it was in
1979. The most acidic water had a pH of 4.5, a reading seen infrequently in
nature. Further, the study considered only the lab effects of those factors on
the eggs of one species. In the wild, each of the many species of amphibian
would react differently at each stage of life to these and other stresses.
Acid rain can send pH levels as low as between 4.6 and 5.4 in lakes in the
eastern U.S., Canada, and Europe, research has shown. Other workers have found
pH levels of 4.5 in northern California and 5.0 in the Rocky Mountains. In
addition, surges of acidified water may occur in mountainous areas when snow
melts in the spring. High-elevation frogs breed in that season - a time of
year when UV radiation is near its peak, the UCSC team notes.
Soule hopes to work with a molecular biologist to explore why the pH-UV double
whammy harms the eggs. The team observed that embryos died when their egg
membranes did not expand, a "curling defect" that trapped the growing tadpoles
inside in tight coils.
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